Lactobacillales -> Tanous, C., Chambellon, E., and Yvon, M. “Sequence Analysis of the mobilizable lactococcal plasmid pGdh442 encoding glutamate dehydrogenase activity”. ATCC was used to determine the optimal temperature for storage. The alanine production with change of pH increases drastically over the maximal conversion of other microorganism. Characterization of a Wild, Novel Nisin A-Producing Lactococcus Strain with an L. lactis subsp. “Mucosal delivery of a Pneumococcal vaccine using Lactococcus lactis affords protection against respiratory infection”. However, the growth rate of the cell with the intake of each carbon source is different. Fig 1 showing the PCR amplification of 16sRNA gene of Lactococcus Table 1: Physiological and Biochemical characteristics of Lactococcus garvieae strain Tests Lactococcus garvieae As a result, optimal lactic acid production can be achieved with the immobilized group of L. lactis and continuous operation with controlled pH. The optimal pH at which this species grows. (5). “GAD catalyzes the irreversible decarboxylation of glutamate to gamma-aminobutyric acid” with the glutamate-GABA antiporter (GABA) (4). Lactococcus garvieae. The main metabolism of L. lactis is through the anaerobic pathway, fermentation, which produces lactic acid from the available carbohydrates and is used for industrial food production. These are important food supplies for many people, so extensive research has been done on the microorganism’s metabolic pathway to increase its efficiency for dairy production. 2007. Lactococcus lactis is researched thoroughly and put into many applications. belonging to a recognized group of disease-invoking agents in humans or animals and having an The optimal temperature at which this species grows. 9. Researchers attempt to prove that a mucosal vaccine against Streptococcus pneumoniae using Lactococcus lactis is more effective than vaccination with purified live antigen. 2006. volume 195. p. 185-193. Cold Spring Harbor Laboratory Press. (1), The metabolic pathway of L. lactis can function through aerobic and anaerobic reactions. No = 0, Yes = 1, Is the species biofilm-forming? Nomura, M., Kobayashi, M., Ohmomo, S., and Okamoto, T., “Inactivation of the glutamate decarboxylase gene in Lactococcus lactis subsp. “Optimization of lactic acid production by immobilized Lactococcus lactis IO-1”. Lactococcus lactis is nonpathogenic bacteria. Gram-stain: Negative = 0, Positive = 1, Indeterminate = 2: Found in human microbiome: Microbes that live anywhere in the human body and are not pathogenic to humans (i.e. 1. for which no prophylaxis is known. The database ranks the pathogenicity of species on a scale of 1 to 4 - 1 being not Lactobacillus bulgaricus is gram- positive. All rights reserved. Journal of Bacteriology. These damages can be fixed when cells are grown with oxygen and heme source. Lactococcus lactis is a spherical-shaped, Gram-positive bacterium used widely for industrial production of fermented dairy products such as milk, cheese, and yogurt. Nature Biotechnology. Microbiology. lactis is preferred for making soft cheese while subsp. It is non-motile and non sporulating. No = 0, Yes = 1. To ensure for maximal production, lactate dehydrogenase gene was knocked out to eliminate any competition for pyruvate substrate and cofactors. cremoris is inactivated by a frameshift mutation resulting in a nonfunctioning protein. The increase in performance of L. lactis reveals another industrial advantage for producing amino acid and genetic engineering. Recently, one method of distinguishing between the two subspecies was announced. Under anaerobic reaction, glycolysis breaks down extracellular carbohydrates to pyruvate, then converts pyruvate to lactic acid with the main enzyme ldh, lactate dehydrogenase (6). Streptococcus thermophilus is a Gram-positive spherical to ovoid nonmotile coccus, 0.7–0.9 μm in diameter, occurring in pairs and chains, some of which can be very long. species in 2011. It has several fermentative pathways, but the most important purpose is its property to manufacture dairy product such as cheese and milk. 64.2% of the genes code for known functional proteins and 20.1% of the genes for known protein with unknown function. The gene that encodes GAD in L. lactis subsp. moderate (mesophilic) environments. No = 0, Yes = 1, Extremophiles are organisms that live in extreme environments, as opposed to organisms that live in Appl Environ Microbiol. G ram-positive bacteria have a thick peptidoglycan layer and a thin cell membrane. 2. extended history of safe usage and 4 being a species that can cause a very serious human disease, COGEM released a comprehensive database of pathogenicity assessment of around 2575 bacterial causing human disease) No = 0, Yes = 1, Does the species causes disease in plants? Firmicutes -> They are Gram-positive bacteria. If two far ranges of pH were determined, the average was taken. However, the major downsides to this method consist of the cost of creating batch of immobilized cells and the cost of fermentation medium. “The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. This category includes acidophiles, thermophiles, osmophiles, cremoris is for hard cheese (1). L. lactis is treated to recombine vaccine strains, so the cell can express the vaccine protein PspATIGR4. Aside from its high use in industrial application, Lactococcus lactis can also be found in the wild on plants and within the digestive tract of cows. The isolated colony is gram positive, gelatin negative, catalase negative, oxidase negative, and non-acid fast. 5. Journal of Industrial Microbiology & Biotechnology. Volume 153. p. 1664-1675, From MicrobeWiki, the student-edited microbiology resource, Family: Streptococcaceae - Members of the family Streptococcaceae include Streptococcus bacteria and Lactococcus among others. These organisms were originally classified under the genus Streptococcus, but in 1985, it was assigned to the current genus (3). Lactococcus -> Volume 17. p. 588-592. Issue 39. The result shows that L. lactis has more potential and safety in developing vaccine in human and should be considered to be used against more pathogens. Volume 34. p. 381-391. (7). 4. Volume 42. Not only it is important in dairy production, it also has potential of use as oral vaccine, foreign protein production and metabolite through genetic engineering to manipulate L. lactis in the researchers’ favor (2). Pediatrics International. No = 0, Yes = 1, Does the species causes disease in animals? 1999. Issue 5. p. 731-753. 2007. BMC Microbiology. 2000. If two far ranges of temperatures Furthermore, it has recently been discovered that a functional electron transfer chain in L lactis supports the membrane potential. 10. It consists of 621 reactions and 509 metabolites and requires minimally glucose, arginine, methionine, glutamate and valine for growth. © 2020 The Microbe Directory. If the species was not widely studied, the American Type Culture Collection (ATCC) was used to determine the optimal pH for storage. 6. The Gram stain test shows differences in the cell walls of bacteria. Dairy industries continue to improve the activities and effectiveness of L. lactis by manipulating its environment and cell behavior. 2001. lactis and subsp. They are Gram-positive, spherical bacteria that grow in chains or pairs. Gram-stain: Negative = 0, Positive = 1, Indeterminate = 2: Found in human microbiome: Microbes that live anywhere in the human body and are not pathogenic to humans (i.e. “Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering”. Description and significance. were determined, the average was taken. “Liver abscess due to Lactococcus lactis cremoris”. Lactococcus lactis specializes in lactate dehydrogenase excreting lactic acid, which is used to preserve food and extend food shelf life. Alexander Bolotin, Patrick Wincker, Stéphane Mauger, Olivier Jaillon, Karine Malarme, Jean Weissenbach, S. Dusko Ehrlich, and Alexei Sorokin. Journal of Basic Microbiology. Volume 11. The alaD gene coding for alanine deydrogenase, an enzyme that converts pyruvate into alanine, was inserted into L. lactis chromosome. capable of causing human disease) No = 0, Yes = 1: Plant pathogen: Does the species causes disease in plants? Streptococcaceae -> Brooijmans, R.J.W., Poolman, B., Schuurman-Wolters, G.K., de Vos, W.M., and Hugenholtz, J. Besides from the anaerobic pathway, L. lactis has an aerobic system to assist in its development. Sean B. Hanniffy, Andrew T. Carter, Ed Hitchin, and Jerry M. Wells. They are characterized by low G+C content and are often referred to as lactic acid bacteria . Oliveira, A.P., Nielsen, J., and Förster, J., “Modeling Lactococcus lactis using a genome-scale flux model”. Along with heme source, the presence of membrane bound NADH dehydrogenase oxidizes NADH and increases the cell growth and production of proteins and vitamins. Lactococcus lactis. 2005. However under low oxygen consumption, aerobic pathway is limited due to the low recycle rate of NAD from NADH oxidase. Lactococcus lactis (ou lactocoque lactique) est une bactérie à Gram positif, non mobile, non sporulante, mesurant en moyenne de 0,5 à 1,5 micromètre.Les cellules de ce micro-organisme se regroupent habituellement en paires ou en courtes chaînes et sont communément isolées dans les produits végétaux, céréales, haricots, pois, pomme de terre, etc. Furthermore, there are around 30 genes for transcription and only 3 sigma factor involved in reading the DNA. Are there any known antibiotics that this species is sensitive to? If the species was not widely studied, the In addition, controlling the pH to 7.5, the mutant cell improves the production to 75% alanine. Normally, the uptake of oxygen would affect the fermentation process or even interferes with oxygen reactive substances. Lactate will guide protons out with membrane protein creating the membrane potential necessary for energy production (1). NADH, the cofactor for the lactate dehydrogenase is regenerated into NAD+ to be reused for glycolysis.